Primer Design | PerseaDB

PCR assay design

Design PCR primers with Primer3

Use an exact PerseaDB DNA record or paste a target sequence, choose a product-size interval, and generate candidate primer pairs locally.

Local Primer3 engine detected

Sequences are processed locally by /usr/local/bin/primer3_core. Candidate primers should still be checked for genome-wide specificity with BLAST.

Genome collection

PerseaDB DNA record identifier (recommended)

Enter one exact gene, mRNA, or CDS identifier. Its stored sequence will be loaded automatically.

or paste a sequence

Sequence name

Target DNA sequence

50–20,000 nucleotides; FASTA header and whitespace are accepted. Leave empty when using a PerseaDB record identifier.

PCR product size range